Rabbit anti-GLRX3/PICOT/TXNL2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-072A-T maps to a region between residue 100 to 150 of human Glutaredoxin-3 using the numbering given in entry O76003.2 (GeneID 10539). Antibody was affinity purified using an epitope specific to GLRX3/PICOT/TXNL2 immobilized on solid support.
The epitope recognized by A305-072A maps to a region between residue 100 to 150 of human Glutaredoxin-3 using the numbering given in entry O76003.2 (GeneID 10539).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The gene GLRX3 encodes for the protein, Glutaredoxin-3. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. Glutaredoxin-3 may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer [taken from NCBI Entrez Gene (Gene ID: 10539)].
Alternate Names
GLRX4; glutaredoxin 4; glutaredoxin-3; GRX3; GRX4; PICOT; PKC-interacting cousin of thioredoxin; PKCq-interacting protein; PKC-theta-interacting protein; testicular tissue protein Li 75; thioredoxin-like protein 2; TXNL2; TXNL3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.