Rabbit anti-GMDS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-508A-T maps to a region between residue 322 to 372 of human GDP-mannose 4, 6 dehydratase using the numbering given in entry O60547.1 (GeneID 2762).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to GMDS immobilized on solid support.
The epitope recognized by A305-508A maps to a region between residue 322 to 372 of human GDP-mannose 4, 6 dehydratase using the numbering given in entry O60547.1 (GeneID 2762).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
GDP-mannose 4,6-dehydratase (GMDS) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020) [taken from NCBI Entrez Gene (Gene ID: 2762)].
Alternate Names
GDP-D-mannose dehydratase; GDP-mannose 4,6 dehydratase; GMD; SDR3E1; short chain dehydrogenase/reductase family 3E, member 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.