Rabbit anti-GPKOW Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-625A maps to a region between residue 426 to 476 of human G Patch Domain and KOW Motifs using the numbering given in entry NP_056513.2 (GeneID 27238).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to GPKOW immobilized on solid support.
The epitope recognized by A304-625A-T maps to a region between residue 426 to 476 of human G Patch Domain and KOW Motifs using the numbering given in entry NP_056513.2 (GeneID 27238).
Additional Product Information
GPKOW is a putative RNA-binding protein containing G-patch and KOW (Kyprides, Ouzounis, Woese) domains. GPKOW interacts directly with protein kinase A and protein kinase X and is also found associated with the spliceosom [taken from NCBI Entrez Gene (Gene ID: 27238)].
Alternate Names
G patch domain and KOW motifs-containing protein; GPATC5; G-patch domain and KOW motifs-containing protein; G-patch domain-containing protein 5; GPATCH5; protein MOS2 homolog; Protein T54; Spp2; T54
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.