Rabbit anti-GSK3-beta Recombinant Monoclonal Antibody [BLR223K]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Request Formulation Change
Additional Product Information
Glycogen synthase kinase 3 beta (GSK3beta) is a proline-directed serine-threonine kinase identified as a kinase responsible for the phosphorylation and inactivation of glycogen synthase. GSK3beta is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. In addition to the Wnt-signaling pathway, GSK3beta is also involved in the Hedgehog, Notch and insulin signaling pathways.
Alternate Names
glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.