Rabbit anti-GST-Tag Recombinant Monoclonal Antibody [BLRE05M]
Product Details
Specifications
Immunogen was a recombinant protein representing Escherichia coli Glutathione S-transferase GstA (residues 1-201, WP_000765749.1).
Additional Product Information
GST (glutathione S-transferase) is commonly engineered onto the N- or C- terminus of a protein of interest to create fusion proteins that can be purified, visualized and analyzed via the properties of the GST tag.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.