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Rabbit anti-GST-Tag Recombinant Monoclonal Antibody [BLRE05M]

bethyl
Bethyl Laboratories Catalog # A191-105
Validated
Target:
GST-Tag
Host:
Rabbit
Clonality:
Recombinant Monoclonal
Clone:
BLRE05M
Format:
Whole IgG
Immunogen:
residues 1-201
Isotype:
IgG
Purity:
Purified
100 µl (10 blots)

A191-105
$482.00
Qty:

Product Details

Specifications

Concentration
40 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing Escherichia coli Glutathione S-transferase GstA (residues 1-201, WP_000765749.1).
Country of Origin
USA

Additional Product Information

GST (glutathione S-transferase) is commonly engineered onto the N- or C- terminus of a protein of interest to create fusion proteins that can be purified, visualized and analyzed via the properties of the GST tag.

945758
gstA
glutathione S-transferase GstA
P0A9D2
Glutathione S-transferase GstA

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.