Rabbit anti-GTF2I/TFII-I Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-330A maps to a region between residue 935 and 985 of human general transcription factor II-I using the numbering given in entry NP_127492.1 (GeneID 2969).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to GTF2I/TFII-I immobilized on solid support.
The epitope recognized by A301-330A-T maps to a region between residue 935 and 985 of human general transcription factor II-I using the numbering given in entry NP_127492.1 (GeneID 2969).
Additional Product Information
GTF2I/TFII-I is a multi-functional transcription factor that interacts with the basal transcription machinery and links upstream cellular signals to responsive activator complexes that associate with the C-FOS promoter and other response elements. Haploinsufficiency of GTF2I/TFII-I results in Williams-Beuren syndrome (WBS), a rare developmental disorder characterized by musculo-skeletal and cardiac abnormalities.
Alternate Names
BAP135; BAP-135; Bruton tyrosine kinase-associated protein 135; BTKAP1; BTK-associated protein 135; BTK-associated protein, 135kD; DIWS; general transcription factor II-I; GTFII-I; IB291; SPIN; SRF-Phox1-interacting protein; TFII-I; WBS; WBSCR6; Williams-Beuren syndrome chromosomal region 6 protein; Williams-Beuren syndrome chromosome region 6
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.