Rabbit anti-HEATR1/BAP28 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-082A maps to a region between residue 1200 to 1250 of human HEAT repeat-containing protein 1 using the numbering given in entry Q9H583.3 (GeneID 55127).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HEATR1/BAP28 immobilized on solid support.
The epitope recognized by A305-082A-T maps to a region between residue 1200 to 1250 of human HEAT repeat-containing protein 1 using the numbering given in entry Q9H583.3 (GeneID 55127).
Additional Product Information
HEAT repeat-containing protein 1, encoded by the gene HEATR1, is involved in nucleolar processing of pre-18S ribosomal RNA and ribosome biosynthesis [taken from UniProtKB (Entry: Q9H583)].
Alternate Names
BAP28; HEAT repeat-containing protein 1; protein BAP28; U3 small nucleolar RNA-associated protein 10 homolog; UTP10; UTP10, small subunit (SSU) processome component, homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.