Rabbit anti-HECTD1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
HECT domain-containing protein 1 (HECTD1) is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. HECTD1 may be required for development of the head mesenchyme and neural tube closure [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9ULT8].
Alternate Names
E3 ligase for inhibin receptor; E3 ubiquitin-protein ligase HECTD1; EULIR; HECT domain containing E3 ubiquitin protein ligase 1; HECT domain-containing protein 1; HECT-type E3 ubiquitin transferase HECTD1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.