Rabbit anti-HECTD3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-924A-T maps to a region between residue 811 to 861 of human E3 ubiquitin-protein ligase HECTD3 using the numbering given in entry Q5T447.1 (GeneID 79654).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HECTD3 immobilized on solid support.
The epitope recognized by A304-924A maps to a region between residue 811 to 861 of human E3 ubiquitin-protein ligase HECTD3 using the numbering given in entry Q5T447.1 (GeneID 79654).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. HECTD3 mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP It also,mediatesubiquitination of STX8 (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5T447].
Alternate Names
E3 ubiquitin-protein ligase HECTD3; HECT domain containing E3 ubiquitin protein ligase 3; HECT domain-containing protein 3; HECT-type E3 ubiquitin transferase HECTD3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.