Rabbit anti-HEXIM1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-113A maps to a region between residue 309 and 359 of human Hexamethylene Bis-Acetamide Inducible 1 using the numbering given in entry NP_006451.1 (GeneID 10614).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HEXIM1 immobilized on solid support.
The epitope recognized by A303-113A-T maps to a region between residue 309 and 359 of human Hexamethylene Bis-Acetamide Inducible 1 using the numbering given in entry NP_006451.1 (GeneID 10614).
Additional Product Information
Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA, HEXIM1sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. HEXIM1 may also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O94992].
Alternate Names
cardiac lineage protein 1; CLP1; EDG1; estrogen down-regulated gene 1 protein; hexamethylene bis-acetamide inducible 1; hexamethylene bisacetamide-inducible protein; hexamethylene bis-acetamide-inducible protein 1; hexamethylene-bis-acetamide-inducible transcript 1; hexamthylene bis-acetamide inducible 1; HIS1; HMBA-inducible; MAQ1; menage a quatre 1; menage a quatre protein 1; protein HEXIM1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.