/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-HIS-Tag Recombinant Monoclonal Antibody [BLRE06M]
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Primary Antibodies  

Rabbit anti-HIS-Tag Recombinant Monoclonal Antibody [BLRE06M]

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
HIS-Tag
Applications:
IP, WB
Host:
Rabbit
Clonality:
Recombinant Monoclonal
Clone:
BLRE06M
Format:
Whole IgG
Isotype:
IgG
Purity:
Purified
100 µl (10 blots)

20 µl (2 blots)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications

Concentration
100 µg/ml 1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Request Formulation Change
Production Procedures
Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide representing a 6X HIS tag (HHHHHH).

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.