Rabbit anti-HMOX2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-353A-T maps to a region between residue 1 to 50 of human Heme oxygenase 2 using the numbering given in entry P30519.2 (GeneID 3163).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HMOX2 immobilized on solid support.
The epitope recognized by A305-353A maps to a region between residue 1 to 50 of human Heme oxygenase 2 using the numbering given in entry P30519.2 (GeneID 3163).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances [taken from NCBI Entrez Gene (Gene ID: 3163)].
Alternate Names
heme oxygenase (decycling) 2; heme oxygenase 2; HO-2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.