Rabbit anti-HNF1-alpha Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-054A-T maps to a region between residue 581 to 631 of human HNF1 Homeobox A using the numbering given in entry NP_000536.5 (GeneID 6927).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HNF1-alpha immobilized on solid support.
The epitope recognized by A304-054A maps to a region between residue 581 to 631 of human HNF1 Homeobox A using the numbering given in entry NP_000536.5 (GeneID 6927).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Hepatocyte nuclear factor 1-alpha (HNF1-Alpha) is a transcription factor required for the expression of several liver-specific genes. HNF1-Alpha functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in the HNF1A gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas [taken from NCBI Entrez Gene (Gene ID: 6927)].
Alternate Names
albumin proximal factor; hepatic nuclear factor 1; hepatocyte nuclear factor 1-alpha; HNF1; HNF-1A; HNF-1-alpha; HNF4A; IDDM20; interferon production regulator factor; LFB1; liver-specific transcription factor LF-B1; MODY3; TCF1; TCF-1; Transcription factor 1; transcription factor 1, hepatic; truncated hepatocyte nuclear factor 1 alpha
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.