Rabbit anti-HO-1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-661A-T maps to a region between residue 1 and 50 of human Heme Oxygenase (decycling) 1 using the numbering given in entry NP_002124.1 (GeneID 3162).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HO-1 immobilized on solid support.
The epitope recognized by A303-661A maps to a region between residue 1 and 50 of human Heme Oxygenase (decycling) 1 using the numbering given in entry NP_002124.1 (GeneID 3162).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Heme oxygenase 1 (HO-1) cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P14618].
Alternate Names
bK286B10; heat shock protein, 32-kD; heme oxygenase (decycling) 1; heme oxygenase 1; HMOX1D; HO-1; HSP32
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.