Rabbit anti-HURP/DLG7 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-853A maps to a region between residue 800 and the C-terminus (residue 846) of Human Hepatoma Upregulated Protein/Discs, Large 7 using the numbering given in entry NP_055565.2 (GeneID 9787).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HURP/DLG7 immobilized on solid support.
The epitope recognized by A300-853A-T maps to a region between residue 800 and the C-terminus (residue 846) of Human Hepatoma Upregulated Protein/Discs, Large 7 using the numbering given in entry NP_055565.2 (GeneID 9787).
Additional Product Information
HURP/DLG7 (Hepatoma Up-Regulated Protein/Disks Large Homolog 7) is a member of the MAGUK (membrane-associated guanylate kinase) family of scaffolding proteins. There are seven DLG homologs in mammals that play important roles in regulating proliferation, epithelial cell polarity, cell adhesion, and synapse formation. HURP/DLG7 has been identified as a microtubule-associated protein (MAP) that colocalizes with the mitotic spindle and functions as a mitotic regulator. HURP/DLG7 is required for microtubule attachment and efficient capture of kinetochores, as well as timely chromosome congression. HURP/DLG7 is up-regulated in human cancer cells and may contribute to tumorigenesis.
Alternate Names
DAP-5; Discs large homolog 7; discs large homolog associated protein 5; discs, large homolog 7; disks large-associated protein 5; disks large-associated protein DLG7; DLG7; hepatoma up-regulated protein; HURP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.