Rabbit anti-IARS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-748A maps to a region between residue 1212 to 1262 of human Isoleucyl-tRNA Synthetase using the numbering given in entry NP_002152.2 (GeneID 3376).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IARS immobilized on solid support.
The epitope recognized by A304-748A-T maps to a region between residue 1212 to 1262 of human Isoleucyl-tRNA Synthetase using the numbering given in entry NP_002152.2 (GeneID 3376).
Additional Product Information
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase (IARS) belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis [taken from NCBI Entrez Gene (Gene ID: 3376)].
Alternate Names
GRIDHH; IARS1; ILERS; ILRS; IRS; isoleucine tRNA ligase 1, cytoplasmic; isoleucine--tRNA ligase, cytoplasmic; Isoleucyl-tRNA synthetase; PRO0785
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.