Rabbit anti-IDH2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-096A maps to a region between residue 402 to 452 of human Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial using the numbering given in entry NP_002159.2 (GeneID 3418).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IDH2 immobilized on solid support.
The epitope recognized by A304-096A-T maps to a region between residue 402 to 452 of human Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial using the numbering given in entry NP_002159.2 (GeneID 3418).
Additional Product Information
Isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2) plays a role in intermediary metabolism and energy production. IDH2 may tightly associate or interact with the pyruvate dehydrogenase complex [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P48735].
Alternate Names
D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; isocitrate dehydrogenase NADP, mitochondrial; isocitrate dehydrogenase 2 (NADP+), mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; oxalosuccinate decarboxylase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.