Rabbit anti-IDI1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-536A maps to a region between residue 1 to 50 of human Isopentenyl-diphosphate Delta-isomerase 1 using the numbering given in entry Q13907.2 (GeneID 3422).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IDI1 immobilized on solid support.
The epitope recognized by A305-536A-T maps to a region between residue 1 to 50 of human Isopentenyl-diphosphate Delta-isomerase 1 using the numbering given in entry Q13907.2 (GeneID 3422).
Additional Product Information
IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity [taken from NCBI Entrez Gene (Gene ID: 3422)].
Alternate Names
IPP isomerase 1; IPP1; IPPI1; isopentenyl diphosphate dimethylallyl diphosphate isomerase 1; isopentenyl pyrophosphate isomerase 1; isopentenyl-diphosphate Delta-isomerase 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.