Rabbit anti-IgM Recombinant Monoclonal Antibody [BLR294L]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Request Formulation Change
Immunogen was purified human IgM (P01871).
Additional Product Information
IgM is typically referred to as a pentamer, containing 5 monomers held together by a J chain protein. IgM is found primarily in serum and is the first part of the antibody response to infection.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.