Rabbit anti-IMPA1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-427A-T maps to a region between residue 25 to 75 of human Inositol monophosphatase 1 using the numbering given in entry P29218.1 (GeneID 3612).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IMPA1 immobilized on solid support.
The epitope recognized by A305-427A maps to a region between residue 25 to 75 of human Inositol monophosphatase 1 using the numbering given in entry P29218.1 (GeneID 3612).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Inositol monophosphatase 1 (IMPA1) is an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. IMPA1 shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorde [taken from NCBI Entrez Gene (Gene ID: 3612)].
Alternate Names
D-galactose 1-phosphate phosphatase; IMP; IMP 1; IMPA; IMPase 1; inositol monophosphatase 1; inositol(myo)-1(or 4)-monophosphatase 1; inositol-1(or 4)-monophosphatase 1; lithium-sensitive myo-inositol monophosphatase A1; MRT59; myo-inositol monophosphatase 1; testicular tissue protein Li 94
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.