Rabbit anti-Importin 4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-725A-T maps to a region between residue 900 and 950 of human Importin 4 using the numbering given in entry NP_078934.3 (GeneID 79711).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Importin 4 immobilized on solid support.
The epitope recognized by A302-725A maps to a region between residue 900 and 950 of human Importin 4 using the numbering given in entry NP_078934.3 (GeneID 79711).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Importin 4 mediates the nuclear import of RPS3A. In vitro, it mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Additionally, along with importin 7, importin 4 has been shown to be involved in nuclear import of HIF-1alpha [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/Q8TEX9].
Alternate Names
Imp4; imp4b; importin-4; importin-4b; ran-binding protein 4; ranBP4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.