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Rabbit anti-Intersectin 1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Intersectin 1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 850 and 900
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to Intersectin 1 immobilized on solid support.

The epitope recognized by A303-075A maps to a region between residue 850 and 900 of human Intersectin 1 using the numbering given in entry NP_003015.2 (GeneID 6453).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to Intersectin 1 immobilized on solid support.

The epitope recognized by A303-075A-T maps to a region between residue 850 and 900 of human Intersectin 1 using the numbering given in entry NP_003015.2 (GeneID 6453).
Country of Origin
USA

Additional Product Information

Intersectin 1 is an adapter protein that may provide an indirect link between the endocytic membrane traffic and the actin assembly machinery. Intersectin 1 may regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q15811].

6453
ITSN1
intersectin 1
Q15811
Intersectin-1

Alternate Names

human intersectin-SH3 domain-containing protein SH3P17; intersectin 1 (SH3 domain protein); intersectin 1 short form variant 3; intersectin 1 short form variant, 11; intersectin short variant 12; intersectin-1; ITSN; SH3 domain-containing protein 1A; SH3D1A; SH3P17; Src homology 3 domain-containing protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.