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Rabbit anti-IP3R1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
IP3R1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 2708 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Rat
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to IP3R1 immobilized on solid support.

The epitope recognized by A302-158A maps to a region between residue 2708 and 2758 of human inositol 1, 4,5-trisphosphate receptor type 1 using the numbering given in entry Q14643.2 (GeneID 3708).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to IP3R1 immobilized on solid support.

The epitope recognized by A302-158A-T maps to a region between residue 2708 and 2758 of human inositol 1, 4,5-trisphosphate receptor type 1 using the numbering given in entry Q14643.2 (GeneID 3708).
Country of Origin
USA

Additional Product Information

IP3R1 (inositol 1,4,5-triphosphate receptor type 1) is an intracellular calcium release channel that mediates calcium release from the endoplasmic reticulum. There are 3 mammalian InsP3R receptors: Insp3R1, Insp3R2, and Insp3R3. Defects in the gene which encodes InsP3R1, ITPR1, are the cause of spinocerebellar ataxia type 15 (SCA15).

3708
ITPR1
inositol 1,4,5-trisphosphate receptor type 1
Q14643
Inositol 1,4,5-trisphosphate receptor type 1

Alternate Names

ACV; CLA4; inositol 1,4,5-triphosphate receptor, type 1; inositol 1,4,5-trisphosphate receptor type 1; INSP3R1; IP3 receptor; IP3 receptor isoform 1; IP3R; IP3R 1; IP3R1; PPP1R94; protein phosphatase 1, regulatory subunit 94; SCA15; SCA16; SCA29; type 1 inositol 1,4,5-trisphosphate receptor; type 1 InsP3 receptor

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.