Rabbit anti-IQGAP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-950A maps to a region between residue 1 and 50 of human motif containing GTPase activating protein 1 using the numbering given in entry NP_003861.1 (GeneID 8826).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IQGAP1 immobilized on solid support.
The epitope recognized by A301-950A-T maps to a region between residue 1 and 50 of human motif containing GTPase activating protein 1 using the numbering given in entry NP_003861.1 (GeneID 8826).
Additional Product Information
IQGAP1 (IQ motif containing GTPase activating protein 1) interacts with the Rho-family GTPases, CDC42 and RAC1. It has been shown to localize to sites of cell-cell contact and regulate cadherin-mediated cell-cell adhesion. In addition to its role in cell adhesion, it is also involved in cell polarization and migration.
Alternate Names
HUMORFA01; p195; ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs; SAR1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.