Rabbit anti-IRF1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-378A maps to a region between residue 300 and 329 of mouse Interferon Regulatory Factor 1 using the numbering given in entry NP_032416.1 (GeneID 16362).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IRF1 immobilized on solid support.
The epitope recognized by A303-378A-T maps to a region between residue 300 and 329 of mouse Interferon Regulatory Factor 1 using the numbering given in entry NP_032416.1 (GeneID 16362).
Additional Product Information
Interferon regulatory factor 1 (IRF1) is a member of the IRF family of transcription factors that plays a critical role in immune cell function and development. In addition to binding upstream regulatory regions of the type I IFN gene, IRF1 also regulates expression of MHC lass I, and plays a role in apoptosis.
Alternate Names
AU020929; interferon regulatory factor 1; Irf-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.