Rabbit anti-IRS2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-434A-T maps to a region between residue 1288 and 1338 of human insulin receptor substrate 2 using the numbering given in entry NP_003740.2 (GeneID 8660).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IRS2 immobilized on solid support.
The epitope recognized by A301-434A maps to a region between residue 1288 and 1338 of human insulin receptor substrate 2 using the numbering given in entry NP_003740.2 (GeneID 8660).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
IRS2 (insulin receptor substrate 2) functions as a cytoplasmic link and transducer of insulin receptor (IR) and insulin-like growth factor receptor (IGFR) signaling. As the name implies, IRS2 is a substrate of IR and IGFR tyrosine kinase activity activated by insulin and IGF binding.
Alternate Names
insulin receptor substrate 2; IRS-2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.