Rabbit anti-JAM-C Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-761A-T maps to a region between residue 260 and 310 of human Junctional Adhesion Molecule C using the numbering given in entry NP_116190.3 (GeneID 83700). Antibody was affinity purified using an epitope specific to JAM-C immobilized on solid support.
The epitope recognized by A303-761A maps to a region between residue 260 and 310 of human Junctional Adhesion Molecule C using the numbering given in entry NP_116190.3 (GeneID 83700).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Junctional adhesion molecule C (JAM-C) participates in cell-cell adhesion. JAM-C is a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form of JAM-C is a mediator of angiogenesis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BX67].
Alternate Names
JAM-2; JAM-3; JAMC; JAM-C; Junctional adhesion molecule 3; junctional adhesion molecule C
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.