Rabbit anti-JMJD2B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-477A-T maps to a region between residue 625 and 675 of human jumonji domain containing 2B using the numbering given in entry NP_055830.1 (GeneID 23030).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to JMJD2B immobilized on solid support.
The epitope recognized by A301-477A maps to a region between residue 625 and 675 of human jumonji domain containing 2B using the numbering given in entry NP_055830.1 (GeneID 23030).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
JMJD2B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JMJD2B has been shown to be capable of antagonizing H3K9 trimethylation.
Alternate Names
jmjC domain-containing histone demethylation protein 3B; JMJD2B; jumonji domain containing 2B; jumonji domain-containing protein 2B; lysine (K)-specific demethylase 4B; lysine-specific demethylase 4B; TDRD14B; tudor domain containing 14B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.