Rabbit anti-JMY IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00718 maps to a region between residue 1 and 50 of human junction mediating and regulatory protein using the numbering given in entry NP_689618.3 (GeneID 133746).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to JMY immobilized on solid support.
The epitope recognized by IHC-00718-T maps to a region between residue 1 and 50 of human junction mediating and regulatory protein using the numbering given in entry NP_689618.3 (GeneID 133746).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Junction-mediating and -regulatory protein (JMY) acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In the nucleus, JMY acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300 and increases p53/TP53-dependent transcription and apoptosis suggesting an important role in p53/TP53 stress response such as DNA damage. In the cytoplasm, JMY acts as a nucleation-promoting factor for both branched and un-branched actin filaments. It activates the Arp2/3 complex to induce branched actin filament networks and also catalyzes actin polymerization in the absence of Arp2/3, creating un-branched filaments [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N9B5].
Alternate Names
junction-mediating and -regulatory protein; WAS protein homology region 2 domain containing 1-like 3; WHAMM2; WHDC1L3
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.