Rabbit anti-KARS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-630A maps to a region between residue 550 and the C-terminus (residue 597) of human Lysyl-tRNA Synthetase using the numbering given in entry NP_005539.1 (GeneID 3735).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to KARS immobilized on solid support.
The epitope recognized by A300-630A-T maps to a region between residue 550 and the C-terminus (residue 597) of human Lysyl-tRNA Synthetase using the numbering given in entry NP_005539.1 (GeneID 3735).
Additional Product Information
KARS is an aminoacyl-tRNA synthetase that functions to charge tRNAs with their cognate amino acids.
Alternate Names
CMTRIB; DFNB89; KARS1; KARS2; KRS; lysine tRNA ligase; lysine--tRNA ligase; lysRS; Lysyl-tRNA synthetase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.