Rabbit anti-LAS1L Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-438A maps to a region between residue 684 to 734 of human LAS1-Like using the numbering given in entry NP_112483.1 (GeneID 81887).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to LAS1L immobilized on solid support.
The epitope recognized by A304-438A-T maps to a region between residue 684 to 734 of human LAS1-Like using the numbering given in entry NP_112483.1 (GeneID 81887).
Additional Product Information
LAS1L is involved in the biogenesis of the 60S ribosomal subunit. It is required for maturation of the 28S rRNA. LAS1L functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y4W2].
Alternate Names
dJ475B7.2; Las1-like; protein LAS1 homolog; ribosomal biogenesis protein LAS1L; WTS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.