Rabbit anti-LGN Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-032A maps to a region between residue 627 and 677 of human Mosaic Protein LGN using the numbering given in entry AAH27732.1 (GeneID 29899).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to LGN immobilized on solid support.
The epitope recognized by A303-032A-T maps to a region between residue 627 and 677 of human Mosaic Protein LGN using the numbering given in entry AAH27732.1 (GeneID 29899).
Additional Product Information
LGN (Leu-Gly-Asn repeat-enriched protein) plays an important role in spindle pole orientation. LGN interacts and contributes to the functional activity of G(i) alpha proteins. LGN acts to stabilize the apical complex during neuroblast divisions.
Alternate Names
CMCS; DFNB82; G-protein signalling modulator 2 (AGS3-like, C. elegans); G-protein-signaling modulator 2; LGN; mosaic protein LGN; PINS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.