Rabbit anti-Limkain b1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-903A maps to a region between residue 1625 and 1675 of human Limkain b1 using the numbering given in entry NP_055462.2 (GeneID 9665).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Limkain b1 immobilized on solid support.
The epitope recognized by A303-903A-T maps to a region between residue 1625 and 1675 of human Limkain b1 using the numbering given in entry NP_055462.2 (GeneID 9665).
Additional Product Information
Limkain b1 is an essential regulator of oogenesis required for female meiotic progression. Limkain b1 acts by down-regulating RNA transcripts, either at transcriptional of post-transcriptional level (by similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y4F3].
Alternate Names
KIAA0430; limkain-b1; LKAP; LMKB; meiosis arrest female 1; meiosis arrest female protein 1; meiosis regulator and mRNA stability factor 1; PPP1R34; protein phosphatase 1, regulatory subunit 34
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.