Rabbit anti-Lipin2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-703A-T maps to a region between residue 375 and 425 of human Lipin 2 using the numbering given in entry NP_055461.1 (GeneID 9663).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Lipin2 immobilized on solid support.
The epitope recognized by A303-703A maps to a region between residue 375 and 425 of human Lipin 2 using the numbering given in entry NP_055461.1 (GeneID 9663).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Lipin 2 plays important roles in controlling the metabolism of fatty acids at differents levels. It acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the endoplasmic reticulum membrane. Lipin 2 also acts as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q92539].
Alternate Names
Lipin-2; phosphatidate phosphatase LPIN2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.