Rabbit anti-LYN Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-683A maps to a region between residue 1 and 50 of human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog using the numbering given in entry NP_002341.1 (GeneID 4067).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to LYN immobilized on solid support.
The epitope recognized by A302-683A-T maps to a region between residue 1 and 50 of human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog using the numbering given in entry NP_002341.1 (GeneID 4067).
Additional Product Information
LYN is a tyrosine protein kinase that acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation. LYN acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. It is involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. LYN induces SAPK by a MKK7- and MEKK1-dependent mechanism [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P07948].
Alternate Names
JTK8; lck/Yes-related novel protein tyrosine kinase; p53Lyn; p56Lyn; tyrosine-protein kinase Lyn; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.