Rabbit anti-MAP1B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-447A-T maps to a region between residue 2050 and 2100 of human microtubule-associated protein 1B using the numbering given in entry NP_005900.2 (GeneID 4131).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MAP1B immobilized on solid support.
The epitope recognized by A301-447A maps to a region between residue 2050 and 2100 of human microtubule-associated protein 1B using the numbering given in entry NP_005900.2 (GeneID 4131).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
MAP1B is one of the founding members of the MAP (microtubule-associated protein) family of proteins involved in microtubule assembly. MAP1B is almost exclusively expressed in the developing brain and is critical for axon formation. MAP1B-null mice exhibit an absence of the corpus collosum and impaired axon development. MAP1B is processed by proteolytic cleavage that leads to the generation of a 300 kDa heavy chain and a 32 kDa LC1 light chain.
Alternate Names
FUTSCH; MAP-1B; MAP5; microtubule-associated protein 1B; PPP1R102; protein phosphatase 1, regulatory subunit 102
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.