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Rabbit anti-MARK2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
MARK2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 350 and 400
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to MARK2 immobilized on solid support.

The epitope recognized by A303-135A maps to a region between residue 350 and 400 of human MAP/Microtubule Affinity-Regulating Kinase 2 using the numbering given in entry NP_001034558.2 (GeneID 2011).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to MARK2 immobilized on solid support.

The epitope recognized by A303-135A-T maps to a region between residue 350 and 400 of human MAP/Microtubule Affinity-Regulating Kinase 2 using the numbering given in entry NP_001034558.2 (GeneID 2011).
Country of Origin
USA

Additional Product Information

MAP/microtubule affinity-regulating kinase 2 (MARK2) is a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes [taken from NCBI Entrez Gene (Gene ID: 2011)].

2011
MARK2
microtubule affinity regulating kinase 2
Q7KZI7
Serine/threonine-protein kinase MARK2

Alternate Names

ELKL motif kinase 1; EMK1; EMK-1; MAP/microtubule affinity-regulating kinase 2; PAR-1; PAR1 homolog; PAR1 homolog b; Par1b; Par-1b; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK; serine/threonine-protein kinase MARK2; testicular tissue protein Li 117

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.