Rabbit anti-MARS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-961A maps to a region between residue 650 to 700 of human Methionyl-tRNA Synthetase using the numbering given in entry NP_004981.2 (GeneID 4141).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MARS immobilized on solid support.
The epitope recognized by A303-961A-T maps to a region between residue 650 to 700 of human Methionyl-tRNA Synthetase using the numbering given in entry NP_004981.2 (GeneID 4141).
Additional Product Information
Methionyl-tRNA synthetase (MARS) is a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [taken from NCBI Entrez Gene (Gene ID: 4141)].
Alternate Names
CMT2U; cytosolic methionyl-tRNA synthetase; ILFS2; ILLD; methionine--tRNA ligase, cytoplasmic; Methionyl-tRNA synthetase; METRS; MRS; MTRNS; SPG70
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.