Rabbit anti-MDC1 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00075 maps to a region between residue 1375 and 1425 of human Mediator of DNA Damage Checkpoint Protein 1 using the numbering given in entry NP_055456.1 (GeneID 9656). Antibody was affinity purified using an epitope specific to MDC1 immobilized on solid support.
The epitope recognized by IHC-00075-T maps to a region between residue 1375 and 1425 of human Mediator of DNA Damage Checkpoint Protein 1 using the numbering given in entry NP_055456.1 (GeneID 9656).
Additional Product Information
Mediator of DNA damage checkpoint protein 1 (Mdc1) is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. It is also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5PSV9].
Alternate Names
homologue to Drosophila photoreceptor protein calphotin; mediator of DNA damage checkpoint protein 1; NFBD1; nuclear factor with BRCT domains 1
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.