Rabbit anti-MED13L Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-420A maps to a region between residue 550 and 600 of human mediator complex subunit 13-like using the numbering given in entry NP_056150.1 (GeneID 23389).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MED13L immobilized on solid support.
The epitope recognized by A302-420A-T maps to a region between residue 550 and 600 of human mediator complex subunit 13-like using the numbering given in entry NP_056150.1 (GeneID 23389).
Additional Product Information
Mediator complex subunit 13-like is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q71F56].
Alternate Names
Mediator complex subunit 13-like; mediator of RNA polymerase II transcription subunit 13-like; MRFACD; PROSIT240; THRAP2; thyroid hormone receptor-associated protein 2; thyroid hormone receptor-associated protein complex 240 kDa component-like; TRAP240L
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.