Rabbit anti-MED15 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-422A maps to a region between residue 450 and 500 of human mediator complex subunit 15 using the numbering given in entry NP_001003891.1 (GeneID 51586).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MED15 immobilized on solid support.
The epitope recognized by A302-422A-T maps to a region between residue 450 and 500 of human mediator complex subunit 15 using the numbering given in entry NP_001003891.1 (GeneID 51586).
Additional Product Information
Mediator complex subunit 15 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. MED15 is required for cholesterol-dependent gene regulation. It also positively regulates the Nodal signaling pathway. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96RN5].
Alternate Names
activator-recruited cofactor 105 kDa component; activator-recruited cofactor, 105-kD; ARC105; CAG7A; CTG repeat protein 7a; CTG7A; Mediator complex subunit 15; mediator of RNA polymerase II transcription subunit 15; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; PC2-glutamine-rich-associated protein; PCQAP; Positive cofactor 2 glutamine/Q-rich-associated protein; positive cofactor 2, glutamine/Q-rich-associated protein; TIG1; TIG-1; TNRC7; TPA inducible gene-1; TPA inducible protein; TPA-inducible gene 1 protein; trinucleotide repeat containing 7; trinucleotide repeat-containing gene 7 protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.