/ Antibodies & Antisera / Primary Antibodies / Rabbit anti-MELK Antibody
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Primary Antibodies  

Rabbit anti-MELK Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Citations (2)
Target:
MELK
Reactivity:
Human
Applications:
IP, WB
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 425 and 475
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Request Formulation Change
Country of Origin
USA

Additional Product Information

Maternal embryonic leucine zipper kinase (MELK) phosphorylates ZNF622 and may contribute to its redirection to the nucleus. MELK may be involved in the inhibition of spliceosome assembly during mitosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q14680].

9833
MELK
maternal embryonic leucine zipper kinase
Q14680
Maternal embryonic leucine zipper kinase

Alternate Names

hMELK; HPK38; maternal embryonic leucine zipper kinase; pEg3 kinase; protein kinase Eg3; protein kinase PK38; tyrosine-protein kinase MELK

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.