Rabbit anti-MEPCE Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-184A maps to a region between residue 200 to 250 of human Methylphosphate Capping Enzyme using the numbering given in entry NP_062552.2 (GeneID 56257).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MEPCE immobilized on solid support.
The epitope recognized by A304-184A-T maps to a region between residue 200 to 250 of human Methylphosphate Capping Enzyme using the numbering given in entry NP_062552.2 (GeneID 56257).
Additional Product Information
7SK snRNA methylphosphate capping enzyme (MEPC) is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q7L2J0].
Alternate Names
7SK snRNA methylphosphate capping enzyme; BCDIN3; bicoid-interacting protein 3 homolog; bin3 homolog; bin3, bicoid-interacting 3, homolog; MePCE
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.