Rabbit anti-MID1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-227A-T maps to a region between residue 617 and 667 of human midline 1 using the numbering given in entry NP_000372.1 (GeneID 4281).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MID1 immobilized on solid support.
The epitope recognized by A302-227A maps to a region between residue 617 and 667 of human midline 1 using the numbering given in entry NP_000372.1 (GeneID 4281).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Defects in the Midline-1 (MID1) gene are the cause of Opitz syndrome, an X-linked recessive disorder characterized by developmental defects. MID1 is a microtubule-associated protein (MAP) with E3 ubiquitin ligase activity. MID1 has been shown to target protein phosphatases 2A (PP2Ac) on microtubules for degradation. MID1 association with PP2A has been proposed to regulate microtubule stabilization for cellular processes such as cell division and migration.
Alternate Names
BBBG1; E3 ubiquitin-protein ligase Midline-1; FXY; GBBB1; MIDIN; midline 1 RING finger protein; OGS1; Opitz/BBB syndrome; OS; OSX; putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1; RNF59; TRIM18; tripartite motif protein TRIM18; tripartite motif-containing protein 18; XPRF; zinc finger on X and Y, mouse, homolog of; ZNFXY
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).