Rabbit anti-MIER3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-194A maps to a region between residue 400 and 450 of human Mesoderm Induction Early Response 1, Family Member 3 using the numbering given in entry CAL38471.1 (GeneID 166968).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MIER3 immobilized on solid support.
The epitope recognized by A303-194A-T maps to a region between residue 400 and 450 of human Mesoderm Induction Early Response 1, Family Member 3 using the numbering given in entry CAL38471.1 (GeneID 166968).
Additional Product Information
The product of the MIER3 (mesoderm induction early response protein 3) gene contains an ELM2 (Egl-27 and MTA1 homology 2) domain as well as a SANT (SWI3, ADA2, N-CoR and TFIIIB) DNA binding domain. The MIER3 protein is an uncharacterized protein proposed to function as a transcriptional regulator.
Alternate Names
mesoderm induction early response 1, family member 3; mesoderm induction early response protein 3; mi-er3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.