Rabbit anti-MIS12 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-776A maps to a region between residue 150 and the C-terminus (residue 205) of human MIS12 homolog using the numbering given in entry NP_076944.1 (GeneID 79003).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MIS12 immobilized on solid support.
The epitope recognized by A300-776A-T maps to a region between residue 150 and the C-terminus (residue 205) of human MIS12 homolog using the numbering given in entry NP_076944.1 (GeneID 79003).
Additional Product Information
MIS12 is an inner kinetochore protein found in a complex with Dsn1, Nnf1, and Nsl1 that functions in kinetochore assembly. This MIS12 complex has been demonstrated to be required for proper chromosome alignment and cell cycle progression.
Alternate Names
2510025F08Rik; hMis12; homolog of yeast Mis12; KNTC2AP; MIS12 homolog; MIS12, MIND kinetochore complex component, homolog; MTW1; protein MIS12 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.