Rabbit anti-MK5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-612A maps to a region between residue 423 and 473 of human mitogen-activated protein kinase-activated protein kinase 5 using the numbering given in entry NP_620777.1 (GeneID 8550).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MK5 immobilized on solid support.
The epitope recognized by A302-612A-T maps to a region between residue 423 and 473 of human mitogen-activated protein kinase-activated protein kinase 5 using the numbering given in entry NP_620777.1 (GeneID 8550).
Additional Product Information
MK5 (MAP kinase-activated protein kinase 5) is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. [taken from NCBI Entrez Gene (GeneID: 8550)].
Alternate Names
MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK5; MK-5; p38-regulated/activated protein kinase; PRAK
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.