Rabbit anti-MMS21 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-126A maps to a region between residue 1 to 50 of human Non-SMC Element 2 using the numbering given in entry NP_775956.1 (GeneID 286053).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MMS21 immobilized on solid support.
The epitope recognized by A304-126A-T maps to a region between residue 1 to 50 of human Non-SMC Element 2 using the numbering given in entry NP_775956.1 (GeneID 286053).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The human MMS21 (Methyl Methanesulfonate-Sensitivity protein 21) homolog is also known as E3 SUMO-protein ligase NSE2. MMS21 is an E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96MF7].
Alternate Names
C8orf36; E3 SUMO-protein ligase NSE2; E3 SUMO-protein transferase NSE2; hMMS21; methyl methanesulfonate sensitivity gene 21; MMS21; MMS21 homolog; Non-SMC element 2 homolog; non-SMC element 2, MMS21 homolog; non-structural maintenance of chromosomes element 2 homolog; NSE2; NSMCE2/PVT1 fusion; PVT1/NSMCE2 fusion; zinc finger, MIZ-type containing 7; ZMIZ7
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.