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Rabbit anti-MMS21 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
MMS21
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to MMS21 immobilized on solid support.

The epitope recognized by A304-126A maps to a region between residue 1 to 50 of human Non-SMC Element 2 using the numbering given in entry NP_775956.1 (GeneID 286053).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to MMS21 immobilized on solid support.

The epitope recognized by A304-126A-T maps to a region between residue 1 to 50 of human Non-SMC Element 2 using the numbering given in entry NP_775956.1 (GeneID 286053).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

The human MMS21 (Methyl Methanesulfonate-Sensitivity protein 21) homolog is also known as E3 SUMO-protein ligase NSE2. MMS21 is an E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96MF7].

286053
NSMCE2
NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase
Q96MF7
E3 SUMO-protein ligase NSE2

Alternate Names

C8orf36; E3 SUMO-protein ligase NSE2; E3 SUMO-protein transferase NSE2; hMMS21; methyl methanesulfonate sensitivity gene 21; MMS21; MMS21 homolog; Non-SMC element 2 homolog; non-SMC element 2, MMS21 homolog; non-structural maintenance of chromosomes element 2 homolog; NSE2; NSMCE2/PVT1 fusion; PVT1/NSMCE2 fusion; zinc finger, MIZ-type containing 7; ZMIZ7

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.