Rabbit anti-MPRIP/M-RIP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-822A maps to a region between residue 550 to 600 of human Myosin phosphatase Rho-interacting protein using the numbering given in entry Q6WCQ1.3 (GeneID 23164).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MPRIP/M-RIP immobilized on solid support.
The epitope recognized by A304-822A-T maps to a region between residue 550 to 600 of human Myosin phosphatase Rho-interacting protein using the numbering given in entry Q6WCQ1.3 (GeneID 23164).
Additional Product Information
MPRIP targets myosin phosphatase to the actin cytoskeleton. It is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MPRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6WCQ1].
Alternate Names
MRIP; M-RIP; myosin phosphatase Rho-interacting protein; p116Rip; Rho interacting protein 3; Rho-interacting protein 3; RHOIP3; RIP3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.