Rabbit anti-MSK1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-747A maps to a region between residue 752 and 802 of human Mitogen- and Stress-activated Protein Kinase 1 using the numbering given in entry NP_004746.2 (GeneID 9252).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MSK1 immobilized on solid support.
The epitope recognized by A302-747A-T maps to a region between residue 752 and 802 of human Mitogen- and Stress-activated Protein Kinase 1 using the numbering given in entry NP_004746.2 (GeneID 9252).
Additional Product Information
MSK1 is a serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). MSK1 Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. MSK1 also phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75582].
Alternate Names
90 kDa ribosomal protein S6 kinase 5; MSK1; MSPK1; nuclear mitogen- and stress-activated protein kinase 1; ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLPK; RSKL; RSK-like protein kinase; S6K-alpha-5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.